8 Questions 17 Answers 0 Followers
Questions related from O. Marte
I'm seeing a second call inside most peaks of my DNA sequencing data. This second (smaller) peak is exactly the next base called. Has any one seen this before? Is this a problem with primers? I'm...
17 April 2017 7,381 11 View
Hello, I am designing a Taqman assay and have used ABI's Primer Express software to design the primers and probe. I've also had a bioinformatician check that the sequences are in conserved areas...
17 March 2017 4,655 1 View
There seems to be salt in my RNA after extraction. I proceeded to perform a cleanup step and while the quality (260/280) improved, the 260/230 remains low. I do not have more sample to...
04 May 2016 1,075 5 View
Would freezing the blood in Trizol enough?
09 March 2016 8,710 13 View
I'm trying to increase sensitivity (detection) for a gene expression assay. The more RNA or cDNA I add the more detection I get. I need to be able to detect at really low parasitemia, so the...
01 July 2015 3,278 5 View
I have whole blood and want to isolate rbc. I've come across some methods that isolate wbc, but haven't been able to find one for the collection of rbc. Any help would be appreciated.
24 April 2015 7,312 8 View
The SYBR Green kit has the extension at 72C for 30 seconds. Should the time change if I decrease the temp?
10 March 2015 4,629 9 View
I need to run a gel before sequencing it. What percent gel should I run it on, and what size ladder is adequate?
30 January 2015 9,625 2 View
