Nyalash Ahmad

2 Questions 3 Answers 0 Followers

Questions related from Nyalash Ahmad

I am working on Salmonella Gallinarum ghost formation by E lysis gene expression. Can I use pET40b(+) for protein expression?

E gene that I customized was inserted in pUCIDT-amp which carries lac promotor. After transformation and induction with 1mM IPTG there were no lysis at all. both induced and control cultures...

29 March 2022 4,227 4 View

How long MgCl2 and CaCl2 solutions could be saved for chemically competent cells formation?

I have been working on bacterial transformation by heat shock method. For preparing chemically competent cells, I prepare 0.1 M Calcium chloride, 0.1 M Magnesium Chloride and 15% glycerol in...

03 February 2022 7,705 1 View