I am trying to separate phosphorylation of JAB1 using phos-tag SDS-PAGE. For the phos-tag SDS-PAGE can I use lysate?

For this experiment I am using two lysates. First one has phosphatase activity and other one does not have the phosphatase activity. I wanted to see the affect of phosphorylation in JAB1. For this...

23 January 2018 4,720 3 View