Nuska Ruffin

3 Questions 3 Answers 0 Followers

Questions related from Nuska Ruffin

How can DEAE-cellulose column be used for purification of an unknown enzyme?

Im planning on using Ion Exchange Chromagraphy by DEAE-cellulose column for purifying an unknown cellulase from soil bacteria. How do i decide on the pH of the column buffer if protein pI is...

12 October 2020 4,051 3 View

Explanations for a white cloudy appearance after the addition of Phenol Chloroform Isoamyl Alcohol (25:24:1) as part of CTAB protocol for bacteria?

I am using a ctab protocol to isolate DNA from cellulose degrading bacteria. After the steps of proteinase K, SDS and CTAB additions and the respective incubation and centrifugal periods, i...

17 August 2020 3,412 5 View

What statistical tests can i use to analyse phytochemical concentrations based on scores?

The concentrations i have are identified as 1-5 rank scores according to the color intensities. How do i use these to statistically analyse the data? (1 being the least and 5 the highest).

12 December 2018 9,482 7 View