Is it normal for 3T3-L1 cells to clump or peel off after removing media and add Q5 media?

I have recently started working with 3T3-L1 cells. After incubate the cells in 96-well plates for 24 hours, I change the media (by suctioning) and add Q5 media. But when I observed the cells...

03 August 2023 6,541 2 View