Nurul Kamal

4 Questions 4 Answers 0 Followers

Questions related from Nurul Kamal

Is this FBS debris in my cell media?

Dear all, I prepared my cell media (EMEM+10%FBS) and another cell media (EMEM+2%FBS+1% Pen/Strep). Incubate the aliquot of the media in 37°C for a few days and saw these. They are not moving and...

25 March 2024 7,379 3 View

Can I store diluted template to be used for QPCR in the lab fridge before running it?

I have prepared a series of diluted template to be used to generate standards but I have not yet add in mastermix as our thermocycler is booked fully so I planned to run the samples later. Can...

01 March 2023 1,190 2 View

Do I have to repeat the whole procedure of generating standard curve if my R value is poor?

Hello everyone, I'm new to Real-Time PCR and I'd like your insight on this. I have generated standard curve by doing 1:10 serial dilution in triplicate. However, the result obtained, of which R...

26 January 2023 3,762 3 View

How do you do a validity test for newly synthesized primers using a conventional PCR?

Hi, Recently I synthesized a new primer (from a published journal) to be used for qpcr analysis. However, if I want to do a run test to validate the primer, how do I do so using a conventional...

23 November 2022 1,426 4 View