Nursena Kayabasi

5 Questions 8 Answers 0 Followers

Questions related from Nursena Kayabasi

How can I find the possible function of CpG islands based on their location within the gene of interest?

I'm doing a homework about finding the CpG islands within a gene of our interest. I picked the FXN gene and found 11 possible CpG islands using this website http://dbcat.cgm.ntu.edu.tw/. I'm also...

19 March 2022 6,359 3 View

Should I use mRNA or genomic DNA sequence to design primers that will be used for both Real Time PCR and Reverse Transcriptase-qPCR?

I need to design a set of primers that will be used for Reverse Transcriptase PCR, and Real Time PCR. I want to use the same primer set for both procedures. Should I use the mRNA sequence as...

23 March 2021 8,835 3 View

Which sequence can be used to design a primer set for DNA detection in qPCR and RNA detection in RT-qPCR? Genomic DNA or mRNA sequences?

I would like to design a primer-probe for DNA detection in qPCR and RNA detection for RT-qPCR. Which sequence should I use? Genomic DNA or mRNA? Please note that I want to use the same primer set...

23 March 2021 7,886 3 View

Designing a primer set that will detect both RNA and DNA?

I'm trying to design a primer set from the human COL1A1 gene. I want to use the same primer set to perform PCR whether I'm using DNA sample or RNA sample obtained from a patient. Should I put the...

23 March 2021 1,254 3 View

How can I design a pair of primers that will both amplify DNA and RNA? What should I pay attention to?

I was assigned to design a pair of primers (one forward, one reverse primer) for the COL1A1 gene with a product size of 400-800 bp. And I need to be able to perform PCR using these primers for...

19 March 2021 8,163 10 View