Nurin Jazlina

4 Questions 5 Answers 0 Followers

Questions related from Nurin Jazlina

My PCR product is showing smears, what are the possible causes?

I ran a PCR reaction and it gave good result during the trial run. However, once I ran the same PCR reaction on all of the other samples, there are smears and appearance of non-specific bands. I'm...

10 March 2024 1,127 9 View

Does anyone have any experience/knowledge of Trichomonas tenax axenic cultivation?

I'm going to do an axenic cultivation of T. tenax from the human oral cavity and I'm having some trouble in finding the suitable culture media for T. tenax. It is found that T. tenax is refractory...

10 May 2023 1,760 0 View

What is the differences of using beta-tubulin gene and srRNA as primer for PCR?

Is there any significance in term of sensitivity/specificity?

28 April 2023 6,054 6 View

Protein extraction from saliva samples

Has anyone had success extracting protein from saliva for LFQ (label-free quantification) using TEAB buffer and 10% SDS? If so, could you share your experience or any protocols that worked well...

01 January 1970 6,544 0 View