Why in standard curve qPCR the most concentrate cDNA sample doesn't work and serial dilutions are correct?

Hi! I did a standard curve of my gene of interest. For this, I started with 100ng of cDNA and did serial dilutions (1:10) of this sample 3 steps. The Ct of this samples are: 100ng --> 32,62...

11 November 2019 5,338 3 View