Nuria Roldan

3 Questions 5 Answers 0 Followers

Questions related from Nuria Roldan

Can we use non-HLA matched epithelial and immune cells for co-culture?

We are co-culturing primary alveolar cells with PBMCs, the cells share the same environment but they are not in physical contact. Depending on the experiment number, PBMCs get activated and...

19 November 2018 8,528 7 View

Anybody has perfused PBMCs using plastic tubes (stickiness issues)?

We are trying to perfuse PBMCs through a microchip in which we have seeded endothelial cells. We load the PBMCs in a syringe and using a syringe pump, we want to perfuse the PBMC through the tubes...

03 April 2018 1,292 3 View

Does anybody know why in the last step of MSP1D1 protein purification this protein precipitates?

I am working on nanodiscs using MSP1D1 as scaffold protein. The procedure of MSP1D1 purification is well stablished but we have found out that during dialysis to remove imidazol in the last step...

06 October 2014 8,927 5 View