Nuri Acevedo

3 Questions 3 Answers 0 Followers

Questions related from Nuri Acevedo

Unable to form Gigaseal during whole-cell patch clamp of brain microvasculature endothelial cells from rat's cortex. Any suggestion?

Hi, I'm trying to record brain microvasculature endothelial cells isolated from adult rat brains, maintained in culture, and recorded during passages 3 to 5. The cells are dissociated with trypsin...

22 October 2024 2,749 2 View

What can I change if I'm not getting colonies with my Gibson assembly product?

I'm assembling one PCR fragment (4.2 kb) with my vector, pECFP (4.8 kb), and transforming them into NEB 5-alpha Competent E. coli cells. Both the fragment and the vector were purified using the...

05 March 2024 1,136 5 View

What happened with my DNA extraction?

Hi, I was trying to extract DNA with TRIzol. When I added chloroform to my sample with TRIzol and after centrifugate it, my phases were inverted. (Photo below) -De antemano muchas gracias

30 August 2022 2,592 4 View