Nur Sakinah Harun

2 Questions 2 Answers 0 Followers

Questions related from Nur Sakinah Harun

How do I confirm the succesful rate of preadipocytes transfection with a His-Tagged expression vector?

I'm going to transiently transfect primary pre-adipocytes with His-tagged plasmid DNA [pcDNA3.1(+)] containing ADIPOQ gene with a SNP to study the gene and protein expression by using FuGENE 4K...

27 November 2023 8,917 1 View

What is the best transfection reagent (FuGENE 4K / Lipofectamine 3000) for pre-adipocytes? And what the best method to detect succesful transfection?

I'm going to transiently transfect subcutaneous pre-adipocytes with His-tagged plasmid DNA [pcDNA3.1(+)] containing ADIPOQ gene with a SNP to study the adiponectin expression. Can any expert...

19 January 2023 8,128 3 View