Nunzio Francesco Franco

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Questions related from Nunzio Francesco Franco

From flow cytometry analysis, can anyone suggest if these cells are viable monocytes?

I have stimulated whole blood with LPS and the cells on the top gate (very high SSC) show up regulation of the intracellular protein i am investigating. The SSC looks unusual but as you can see...

08 August 2015 7,587 10 View

Using flow cytometry and CD14,CD16,CD45 and HLA-DR, do monocyte subset change after whole blood stimulation with LPS/IFNg when plotted on CD14vsCD16?

I am stimulating whole blood directly after collection with LPS and IFNg (trying to avoid fickle-paque). Using the above mentioned Ab, when staining the blood at 0 timepoint, the subset look very...

07 July 2015 2,292 7 View