Nuno Santos Leal

3 Questions 4 Answers 0 Followers

Questions related from Nuno Santos Leal

Is it possible to quantify protein levels from tissue material fixed with glutaraldehyde and formaldehyde?

Hi everyone, Me and a colleague of mine perfused animals with 2% glutaraldehyde, 1% formaldehyde in 0.1 M phosphate buffer. We then hemisected the brain and the right hemisphere was kept in the...

03 July 2018 1,508 3 View

Does anyone have seen several bands on a blot when checking for Mfn2 in humans cells?

Hej! I am currently working with Mitofusin-2 protein and I have been using an antibody from Sigma that binds to the N-terminal of the protein and most of the times I see only one band on my WB but...

13 December 2014 8,502 2 View

What is the difference between Smooth ER and Rough ER?

I know that the big difference is the presence and absence of ribosomes and that both types have different function but what is the difference molecularly-wise between these two structures? Are...

14 April 2014 7,774 2 View