Nuno Santos Leal

4 Questions 10 Answers 0 Followers

Questions related from Nuno Santos Leal

Is it possible to quantify protein levels from tissue material fixed with glutaraldehyde and formaldehyde?

Hi everyone, Me and a colleague of mine perfused animals with 2% glutaraldehyde, 1% formaldehyde in 0.1 M phosphate buffer. We then hemisected the brain and the right hemisphere was kept in the...

03 July 2018 1,618 3 View

Does anyone have seen several bands on a blot when checking for Mfn2 in humans cells?

Hej! I am currently working with Mitofusin-2 protein and I have been using an antibody from Sigma that binds to the N-terminal of the protein and most of the times I see only one band on my WB but...

13 December 2014 8,546 2 View

Where is APP cleaved? Where is Beta and Gama-secretase localized within the cell?

Several ideas and suggestions have emerged lately to try to explain where APP is cleaved within the cell. It looks like the first cleavage (by Beta secretase) is thought to be mostly at Plasma...

16 May 2014 8,453 3 View

What is the difference between Smooth ER and Rough ER?

I know that the big difference is the presence and absence of ribosomes and that both types have different function but what is the difference molecularly-wise between these two structures? Are...

14 April 2014 7,821 2 View