Numan Mughal

4 Questions 9 Answers 0 Followers

Questions related from Numan Mughal

How can I increase the chances of ligation?

I am facing difficulties in cloning a 2.5kb DNA fragment into a vector (pGreen0029). My plates were designed as +ive = pGreen0029, -ive = competent cells (cc), L1-3 = ligation reactions performed...

25 May 2021 8,520 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

26 February 2021 3,479 7 View

Does plant tissue culture media pH change significantly, if I add all the ingredients of MS media except gelling agent and keep it on 4C overnight?

If I add the following ingredients of MS media 1- MS salts (+ Macro, micro and vitamins) 2- Sucrose and adjust the pH of media 5.8 and keep it overnight at 4 C? Can any body tell me that pH of...

16 July 2019 7,999 6 View

What would you suggest me before running PCR reaction?

Dear Scientists, Hopefully, you all are well. My work is on pathogen related gene family in plants. I am performing PCR from last 4 months and getting results on and off. I am wondering that...

24 June 2019 7,288 8 View