Norbert Volkmar

4 Questions 10 Answers 0 Followers

Questions related from Norbert Volkmar

Does anyone have experience in preparing strongly adherent cells (U2OS) for FACS without using acute dissociation treatments such as trypsin?

Hi, I need to perform a FACS experiment on strongly adherent cells (U2OS). Does anyone have experience in preparing these cells for FACS without using acute dissociation treatments such as...

11 November 2014 7,377 6 View

Problem with Blue Native PAGE: Why is the transfer incomplete?

I am experiencing problems with one-dimensional Blue Native PAGE: It seems that proteins in the rage of 300 - 600 kDa are not properly transferred onto the PVDF membrane during Western Blotting of...

07 July 2014 8,551 6 View

Can the pcDNA5FRT/TO vector be used for transient overexpression of a gene of interest?

Hi, I have my gene of interest in pcDNA5/FRT/TO and would like to generate a stable cell line. Before generating the stable line, however, I would like to test expression by transient...

07 July 2014 7,787 3 View

Mammalian expression vector for isolation of native protein complexes?

Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...

07 July 2014 5,041 11 View