Noor Nordin

2 Questions 6 Answers 0 Followers

Questions related from Noor Nordin

Why, in MTT assay, do we measure the optical density (OD) at two different wavelengths?

I've been reading through some protocols and also come across other journals which only take a reading at a 570nm wavelength. Is it wrong (or is the data valid) if someone hasn't read the...

30 January 2013 7,955 24 View

Is it necessary to remove MTT medium prior to DMSO addition?

After 4h incubation with MTT reagent, some people remove the MTT medium before adding the DMSO, while others would leave the MTT medium prior to DMSO addition. Can this really be done (leaving the...

23 January 2013 8,053 68 View