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Questions related from Noha M. Hosny
I have a c18 column with a high pressure (2400 psi at flow rate 1 ml/min, acetonitrile:water 50:50). 1_ we use solvents for HPLC and were filtered by nylon paper. 2_ we tried 100% ACN and pressure...
05 May 2018 1,274 16 View
I used c18 and platinum columns and all drugs appeared at the same retention time. By using different mobile phases, aqueous:organic 90:10 v/v. Acetate buffer pH 4 was used also but no separation...
12 December 2017 6,986 7 View
I tried c18 and platinum columns also ion pair and B_CD were used, no separation was observed. Mobile phase , 90:10 v/v aqueous to organic solvent with and without buffer were not effective.
12 December 2017 7,462 2 View
How can i prevent peak fronting of acidic compound in TLC?
03 March 2016 9,651 4 View
I am using acetonitrile and water as a mobile system and C18 column for separation of some drugs, but the obtained peaks were broad and tailed. How can I improve the shape of these peaks?
11 November 2013 2,302 31 View
