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Hi, I usually use sucrose lysis buffer ( 0.6 M sucrose, 0.02 M Tris base, 0.01 M MgCl2 and 2% triton) for RBCs lysis in DNA extraction. After initial RBCs lysis and washing, a pellet of WBCs is...
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when a patient receives frequent transfusions (every 4-6 weeks) how long do his CBC and hemoglobin electrophoresis take to return to baseline or to reach a plateau
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if i want to measure some microRNAs from the plasma of a transfusion dependant patient, what is the minimum period after the last blood transfusion in order to take a representative blood sample
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