Is the annealing temp. for a pair of primers is set according to qpcr kit itself or the primer itself? and which is better ... 2 or 3 step- reaction?

Hi, I am working on gene expression, and want to ask about 2 issues ... I am using SYBR green, but I am confused because each kit has its own specific Ta, while the primer itself has a different...

24 October 2019 10,027 3 View