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We are facing a problem in different groups in our institute: when we grow E. coli (DH5a) overnight in liquid LB, we have: - no growth at all - sometimes, some tubes show cell growth, some...
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I tried to synthesise an artificial miRNA precursor using T7 transcription (I cloned the sequence of my pre-miRNA with a T7 promoter sequence). I ran the RNA on a gel, but I see a smear for the...
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