Nkemngo Francis Nongley

4 Questions 6 Answers 0 Followers

Questions related from Nkemngo Francis Nongley

BLAST serach for Sequence conservation??

Hello, Please, can anyone kindly assist me with protocol on how to check for protein sequence conservation of amino acids across parasite species of the same genus. Thank you!!

21 September 2018 829 3 View

Performing a BLAST Search??

Hello, Please can anyone indicate a protocol on how to perform a BLAST search to check for sequence homology/identity of a parasite protein if it exist in humans or not. Please, I really need...

21 September 2018 7,491 6 View

Site directed cloning of DNA insert in PET15B VECTOR ?

Good day! Pleas can anyone help me with a protocol for site directed cloning of insert DNA containing XhoI/BamHI restriction fragments on PET15b expression vector containing XhoI/BamHI sites...

22 March 2018 2,456 4 View

Good day, Why post- transformation restriction enzyme digestion didnot give me bands of my plasmid and insert?

I cloned Cryptosporidium parvum LDH gene using PMAL-C2X plasmid for which I had clear colonies after transformation on Amp+ plates. After performing miniprep of some selected colonies, I didn't...

08 March 2018 1,084 3 View