Nivetha Ramesh Babu

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Questions related from Nivetha Ramesh Babu

What kind of stresses do mammalian cells undergo when kept in the same culture plate even after they are fully occupied?

In my recent experiments to determine phosphorylated H2AX protein in response to adding a radio mimetic drug, I have been observing abnormally high levels of the the phosphorylated H2AX in the...

07 July 2018 4,858 4 View

Recommended dose for treating HMEC cells (Breast epithelium) with Neocarzinostatin (NCS) to induce DNA damage?

I tried using 100ng/ml media concentration based on the literature from Breast cell lines similar to HMEC. I checked for phosphorylated H2AX and p-chk2 to quantify DNA damage through Western...

04 April 2018 8,995 0 View

What is the recommended dose of Zeocin for selecting stably transfect HEK293T cells resistant for zeocin?

I am working with stable transfection of a plasmid resistant for zeocin in HEK293T cells which have previously undergone transfection for another set of plasmid that is puromycin resistant. I read...

02 February 2017 8,348 3 View