Do any one provide the procedure/thermal cycle pattern to be followed for Loop Mediated Isothermal amplification in real time detection?

I am using the ingredients of 20mM Tris HCl, 10mM (NH4)2SO4, 50 mM KCl, 8mM MgSO4, 0,1 % Tween, 0.8 M Betaine, 1.4 mM dNTPs mix, FIP/BIP primers 1.6 µM, F3/B3 0.2 µM, Loop F/Loop B 0.4 µM, Bst...

09 February 2022 3,407 3 View