Nitish Rai

3 Questions 3 Answers 0 Followers

Questions related from Nitish Rai

What is the methodology to differentiate rat PC 12 cell lines with NGF?

I am trying to differentiate PC12 cells in 96 well plate ( 10000/ well) with the help of NGF but my cells kept on dividing even after the addition of NGFand forms a clump so they do not...

05 May 2016 5,646 3 View

What are the drawbacks of autoinduction of culture for protein purification?

What are the drawbacks of Autoinduction of bacterial culture for protein purification? What about the stability of the protein expressed by such methods. Does anyone know about the report that...

12 December 2015 8,285 4 View

Can we keep the cell culture flask for some time after the treatment time is over for the purpose of protein isolation?

I have to do a western blotting for observing the expression of specific protein of interest. Cells were treated for a specific time with a specific substance and incubation time is over. Now can...

10 October 2014 9,538 4 View