Why am I not getting amplification of a 5.6kb gene using primers with restriction sites and additional bases?

I am using Phusion polymerase, with annealing time being 3minutes, genomic DNA as template (conc. range used: 30ng/µL to 50ng/µL). I have run gradient PCR multiple times and tried different other...

30 May 2025 5,114 6 View