Nishant Belugali Nataraj

2 Questions 9 Answers 0 Followers

Questions related from Nishant Belugali Nataraj

ShRNA mediated knockdown inhibiting protein expression but not in the RNA level, what might be the reason?

I did shRNA knockdown of a protein and did WB. I did not see any protein expression.Result is reproducible. Still I tried different antibody from different company, again i don't see any protein...

26 February 2017 8,960 11 View

NO pcr product in crispr clones?

I used 2 gRNA for knockout a specific gene. Isolated single clones.DNA extracted and run the pcr using diff set of primers (100 to 1000bp) either side flanking to target site but i don't see any...

08 June 2016 5,718 9 View