Nisha Peter

2 Questions 3 Answers 0 Followers

Questions related from Nisha Peter

Has anyone done co-transfection with siRNA and the fucci system viral vectors by Thermofisher (Cat: P36238) ?

I'm trying to knockdown my protein in adherent cells and label cells with the fucci system for cell-cycle segmentation using microscopy. My protocol is as follows Day 0 : siRNA...

08 August 2018 4,520 4 View

Is it normal that Flag tagged protein migrates slower than the same protein tagged with HIS-Flag tag?

When my protein was tagged only with the Flag tag , it shows a shift on the western blot. However when I tag it with 6X His and Flag it comes back to the original size. Is this normal? The Flag...

01 June 2015 3,094 6 View