28S, 18S bands of different sizes in RNA denaturing gel. What may have caused this?

I ran RNA denaturing gel (1.2% agarose, 1X HEPES-EDTA buffer and 0.45M formaldehyde). Used 0.5 ug of RNA, 5 ul of loading dye (HEPES-EDTA, bromophenol blue) and 1ul of Etbr in the reaction. After...

13 June 2017 2,974 4 View