Nireekshit Addanki Tirumala

3 Questions 7 Answers 0 Followers

Questions related from Nireekshit Addanki Tirumala

Can any one identify the problem with HeLa cell culture shown in the image.

Recently, I have started seeing some cell clumps in my culture of HeLa cells. I thawed a fresh batch of cells and they also have it. It does not look like a contamination because the media is fine...

02 August 2017 3,771 11 View

In my TIRF microscopy setup, the laser is leaking through filters when I put the objective in the light path. What could be the possible reasons ?

I have a TIRF setup with a 488nm laser. I am using a quad band dichroic (405/488/561/635) in combination with a quad band fluorescence filter (446/523/600/677). When the objective is not present...

07 April 2017 1,173 1 View

How do make sure that siRNA sequence affects only the protein of interest and not anything else ?

I have HeLa cells stably expressing mouse cytoplasmic Dynein heavy chain. I want to check if the mouse dynein is able to compensate for the loss of human dynein. I wish to knockdown the endogenous...

31 March 2017 2,559 3 View