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Recently, I have started seeing some cell clumps in my culture of HeLa cells. I thawed a fresh batch of cells and they also have it. It does not look like a contamination because the media is fine...
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I have a TIRF setup with a 488nm laser. I am using a quad band dichroic (405/488/561/635) in combination with a quad band fluorescence filter (446/523/600/677). When the objective is not present...
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