How to prevent the conversion of Cytochrome P450 into Cytochrome P420 enzyme during isolation from bacterial membrane?

The CYP450 enzyme isolated from bacteria in presence of 20% glycerol, 1mM DTT, 1mM PMSF in 0.1M Potassium phosphate buffer pH 7.4, during assay with carbon monooxide i got the peak at 418nm...

01 January 2019 6,169 2 View