Nils B.

2 Questions 1 Answers 0 Followers

Questions related from Nils B.

Detaching monocytes from plate?

I need help in detaching monocytes from my plate. I'm seeding PBMCs for overnight culture (adding LPS) in a 96 well plate (polystyrene), stain these cells the next day and use FACS to analyse...

30 January 2022 7,332 3 View

Why do I have a loss of monocytes after lps exposure (18 h incubation)?

Hey guys, I am currently stimulating cryo pbmcs for 18 hours with lps (100ng/ml) right after thawing these cells. After my incubation I stain these cells extracellulary for CD14 and CD16 for...

15 December 2021 2,950 2 View