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usually, Trypan blue stain is applied before seeding cells in 96 wells plate and cells are count by hemocytometer. I am confused about how cells will be counted in 96 wells plate by trypan blue...
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I use 3 g dry sample for extraction and get 83 mg dry extract. from 83 mg I used 60 mg for folio-ciocalteu assay and get 60 mg/g TPC. I want to find the % yield of the TPC. anyone please help me...
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