Nilam Sartika

2 Questions 2 Answers 0 Followers

Questions related from Nilam Sartika

Is it possible that my sequencing result different with my RFLP result in gel elecrophoresis?

currently, I am working in PCR-RFLP. I just get my PCR result sequenced, and the result shows that my PCR sequence has the restriction site. But, in electrophoresis agarose, the digested DNA band...

20 April 2017 6,511 6 View

Is it impossible if the result of PCR has two bands? I used mutagenic primer, is it affect the result of the amplification?

I was doing amplification using mutagenic primer, and the result of the PCR in polyacrylamide gel showed two bands. Both were significant bands. I have increasing the annealing temperature and...

29 January 2017 4,946 3 View