Nikolaos Tsopoulidis

4 Questions 3 Answers 0 Followers

Questions related from Nikolaos Tsopoulidis

Which fluorescent proteins to use to calculate ratio?

Hi, I would like to tag two endogenous proteins with a fluorescent tag to calculate their ratio of expression. Preferentially one bright red and one bright green. Which fluorescent proteins...

05 June 2020 5,584 1 View

Protocol for murine CD4+ T cell transduction?

PQCXIX and mCherry for murine T cell transduction any protocol?

18 December 2017 6,007 3 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

Hello, I would like to use s. aureus enterotoxin B (SEB) for conjugate formation of T cells and B cells (live microscopy). I want to use SEB because it is available from SIGMA, whereas SEE is more...

26 July 2017 512 7 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

02 February 2017 7,509 4 View