Nikolaos Mastrodimos

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Questions related from Nikolaos Mastrodimos

Has anyone transformed Agrobacterium with the Gateway vector pAMPAT?

Hello everyone, I am having some trouble in transforming Agrobacterium cells with the Gateway vector pAMPAT. For the time being, I have only tried GV3101 and have not managed to make it work...

01 February 2022 6,334 8 View

Bacterial cell cultures grow fine in solid media (LBA + antibiotics) but they do not grow in liquid media (LB + same antibiotics). Any ideas?

Hi everyone, I work with Pseudomonas fluorescens EtHAn strain and I 'm facing this issue: Transformed EtHAn cells grow normally in solid media (LBA + 3 selective antibiotics), but when I...

27 February 2020 2,579 5 View