Niklas Eckert Elfving

3 Questions 7 Answers 0 Followers

Questions related from Niklas Eckert Elfving

Gravity-Flow SEC for Purification of a 200 kDa Protein in E. coli – Column Height Suggestions?

Hi everyone, I'm working with a protein of interest (POI) that is approximately 200 kDa, which I'm producing in E. coli. After cell lysis and Strep-tag purification, I can isolate my protein, but...

04 October 2024 6,150 2 View

Can DNA be fluorescently labeled by PCR using primers that carry the fluorophore at the 3'-end of the primer?

Hi! I am to site-specifically label dsDNA parts using PCR with fluorescently labeled primers. For my objective it would be optimal if the primers could carry the fluorophore as close to their...

27 March 2024 9,423 6 View

Digestion with Nde1 and Spe1 with modified pET24(+) plasmid

Hi, I have been trying simultanious digestion of a modified pET24(+) plasmid with Spe1 and Nde1 in a single mixture. The buffers documentation states 100 % efficiency for Nde1 and 75 % efficiency...

01 January 1970 6,787 2 View