I am trying to clone a 1.76 kb gene in pGEM-T easy vector, but all I get is colonies containing self-ligated pGEM-T, where am I going wrong?

I have tried cloning with both PCR purification and gel purification of my amplified PCR product. I have used insert: vector ratio 3:1 and 8:1. I also have difficulty in amplifying my gene...

22 February 2022 2,512 3 View