Nikhil Shah

5 Questions 8 Answers 0 Followers

Questions related from Nikhil Shah

Does anyone know what the ploidy level of the MIN-6 beta cell line is?

doses anyone knoe What is the ploidy level of MIN-6 beta cell line?

14 May 2015 9,487 2 View

What Bioinformatics tool can I use for PCR efficiency check ?

I am looking for tool to evaluate the PCR efficiency for any template/gene. Especially to get an idea about the nature/complexity of gene stretch e.g. Any stable secondary structures, polymerase...

22 April 2015 9,887 3 View

Has anybody worked with quantitative measurement of GFP and RFP?

I want to develop an assay platform for two genes in the same construct. Where GFP will be downstream to one of the gene and RFP for the other one. And finally I want to see the receptor mediated...

23 March 2015 6,692 5 View

How can I digest only one RE site of vector if having two sites of the same REs?

I am using pIRES bicistronic vector for cloning, where it contains two sites of Sma-I in MCS-B region. I have designed cloning primers with sma-I forward and Sal-I reverse. Since another sma-I...

16 March 2015 9,931 8 View

How long can a recombinant CHO cell line survive?

I have cloned one of the gene in CHO cells and generated stable cell line, but after 6-8 passages cells are undergoing morphological changes and decreased activity in in-vitro assays. Can anyone...

04 February 2015 261 8 View