Nikhil Kulkarni

6 Questions 8 Answers 0 Followers

Questions related from Nikhil Kulkarni

RNA digestion in cell culture supernatant?

I want to digest all RNA in cell culture supernatent. I am planning to do a RNAse A treatment . Are there other recommendations for inactivating RNA?

11 March 2017 4,148 2 View

How can I verify drug binding to a ligand (nuclear receptor)?

We are looking at whether a putative drug binds to a nuclear receptor? What experiments can be suggested? We have looked at possible interaction of the drug with the receptor using autodock and...

03 November 2015 4,637 4 View

Can anyone help with our low RNA isolation yield from CD14 MACS purified monocytes?

We have been trying to isolate total RNA from about 1 million MACS purified CD14 monocytes. We get very low quantities of RNA (20 ng/ul in a 30 ul elution volume totalling about 600 ng RNA) from...

11 April 2015 5,684 6 View

What free softwares and/or resources are available in order to learn how to dock a ligand (drug) to a protein?

I want to check whether my drug of interest docks into the active site of a nuclear hormone receptor. For this I need expert assistance me with the analysis.

03 June 2014 2,021 1 View

Does any one have experience with prediction of transcription factor binding sites ?

I want to find putative transscription factor binding sites in the promoter of gene of my interest . I want to know what softwares can be used and if any one can help me with using these softwares...

10 December 2012 6,751 2 View

Change in Ct values over passages in untreated cells?

I have been doing qRT-PCR for TNF gene and I saw that there was a difference in Ct values in the untreated cells over subsequent three passages. When I look at the reference gene beta tubulin it...

18 October 2012 6,622 6 View