Nikhil Kulkarni

3 Questions 5 Answers 0 Followers

Questions related from Nikhil Kulkarni

RNA digestion in cell culture supernatant?

I want to digest all RNA in cell culture supernatent. I am planning to do a RNAse A treatment . Are there other recommendations for inactivating RNA?

11 March 2017 4,107 2 View

What free softwares and/or resources are available in order to learn how to dock a ligand (drug) to a protein?

I want to check whether my drug of interest docks into the active site of a nuclear hormone receptor. For this I need expert assistance me with the analysis.

03 June 2014 1,973 1 View

Change in Ct values over passages in untreated cells?

I have been doing qRT-PCR for TNF gene and I saw that there was a difference in Ct values in the untreated cells over subsequent three passages. When I look at the reference gene beta tubulin it...

18 October 2012 6,570 6 View