Niharika Nag

3 Questions 2 Answers 0 Followers

Questions related from Niharika Nag

Why is my protein concentration increasing after storage at -20°C?

I have a few proteins that I produce recombinantly in E. coli cells. All of them are disordered proteins and are stored in the presence of 4mM DTT at -20°C before use. One of them also contains...

29 November 2023 2,663 2 View

What is the role of 8M urea in protein purification buffer besides solubilization?

Hello, I am trying to express and purify recombinant nuclear pore proteins in E. coli cells. All the published protocols I have found use 8M urea in the lysis buffer and subsequently during...

20 October 2022 4,613 5 View

Web-servers and computational tools to probe IDP-IDP interactions?

Hello, I am looking for good web-servers and computational tools which can help me identify/study/investigate interactions between two or more intrinsically disordered proteins. A reference to...

09 August 2021 8,649 2 View