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Questions related from Nihad H. A. Alothman
EGFR in order to be detected by western blot needs to be separated as a whole non denatured protein so antibody can bind and then can be detected, so SDS can not be used or even beta...
19 July 2016 1,091 5 View
EGFR is a native protein it does not need SDS, I don't know how to prepare the stacking and the resolving gel, should I add SDS, what about the transfer to nitrocellulose membrane should I add SDS...
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