Nigel Aminake Makoah

3 Questions 11 Answers 0 Followers

Questions related from Nigel Aminake Makoah

Has anyone successfully expressed with pCold-I?

I am a first time user and I obtained expression both in Induced and uninduced (even more expression) using BL21 (DE3) after culturing in LB medium and inducing with 1 mM IPTG. It looks very...

10 November 2014 6,250 3 View

Why can I not see induced proteins using two combinations: 1mM IPTG for 4h at 37°C and 1mM IPTG for 14h at 21°C?

I am using pET28b+. Cloning was performed with RE XbaI/NotI and XbaI/HindIII, and expression was done into BL21.

30 October 2014 1,240 7 View

Indirect ELISA using mouse antisera

I have been trying to develop an ELISA assay to identify the presence of the whole parasite in culture by targeting one of its membrane protein. I recently found out that there is no signal...

03 April 2013 9,331 6 View