I want to transfer ~500Kd protein for western blotting using chemiluminescence. Any advice, suggestions for the buffer, voltage, time ? Thanks!

I tried a transfer on PVDF with Tris-Glycine buffer containing 10% methanol and 0.1%SDS at 25V for 16 hrs. My protein ladder (HiMark from Invitrogen) went through so I was not sure of the...

14 August 2017 1,250 2 View