Have you activated CD4+ T cells from OTII mice with OVA peptide in a suspension of whole splenocytes?

I want to activate CD4+ T cells (from OTII mice) in the presence of IL-27 using OVA peptide. I would like to use a suspension of whole splenocytes to do this instead of BMDCs cultured with OVA...

15 September 2014 6,665 4 View