Nicole Mancini

2 Questions 4 Answers 0 Followers

Questions related from Nicole Mancini

Has anyone used the Promega RealTime-Glo Annexin V Apoptosis and Necrosis Kit to study cells infected with bacteria in vitro?

Using this kit, I have found a greatly reduced luminescent signal in my treatment groups that have bacteria, and I am wondering if the bacteria have the ability to alter the kit reagents.

03 March 2018 6,949 4 View

Is it appropriate to western blot for the mitochondrial protein Fis1 in mitochondrial fractions of protein lysates, rather than whole cell lysates?

In the literature I mostly see western blots for the mitochondrial fission receptor protein Fis1 done on whole cell lysates, rather than on isolated mitochondria. I was wondering why this is the...

12 December 2017 647 6 View