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Hi everyone, I am trying to amplify the ITS 1 and 4 regions of Aspergillus flavus. My first three lanes, starting from the left, are A. flavus, I then have 2 lanes of C. albicans as a positive...
23 August 2021 1,464 2 View
I used the TOPO TA Cloning Kit for Sequencing to clone a desired gene into the pCR®4-TOPO® vector. I have been able to confirm the presence of my gene by colony PCR. I then extracted my plasmids...
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