Nick Memmer

3 Questions 6 Answers 0 Followers

Questions related from Nick Memmer

Are smaller, "incomplete" bands on a Q5 mutagenesis gel of concern?

I am attempting a deletion from purified plasmid using the NEB Q5 mutagenesis kit. After mutagenic PCR I ran a gel to attempt to observe the band corresponding to the length of my mutagenized...

02 February 2017 6,455 3 View

Good plate-reader protocol for GFP measurements in S. cerevisiae?

Can anyone recommend a solid protocol for measuring GFP in S. cerevisiae whole cell suspensions using a plate reader? I could use some direction on normalizing, appropriate sample volumes and ODs,...

01 January 2017 7,408 5 View

Has anyone used Thermofisher Y-PER yeast lysis buffer in combination with MagneHis protein purification? Can I just sub Y-Per for the supplied buffer?

I need to purify a His-tagged protein from S. cerevisiae, and the Promega MagneHis system seems well suited to the application. The only problem is that their supplied lysis buffer is intended for...

11 November 2016 2,990 2 View