Nick Schaum

3 Questions 7 Answers 0 Followers

Questions related from Nick Schaum

Why do my amplification curves appear dampened with the Fluidigm gene expression array?

I am using the 96.96 Fluidigm gene expression array with samples from various mouse tissues (not single cell), as well as a set of purchased fetal cDNA. In the attached image, the group of...

14 August 2015 7,714 2 View

Do I need two sets of primers for each gene for the Fluidigm gene expression array?

I have conflicting recommendations from Fluidigm tech support and users as to whether it is worth it to run each gene with two sets of primers. I am running a 96.96 gene expression array on...

24 April 2015 8,777 4 View

How can I avoid RNA degradation from DNase I treatment?

I am extracting RNA from various mouse tissues, including spleen and pancreas, for qPCR with the Fluidigm BioMark 96.96 Dynamic Array. As many others have observed, RNA integrity is compromised...

06 April 2015 6,944 5 View