Nick Brose

3 Questions 2 Answers 0 Followers

Questions related from Nick Brose

What is the proper dosage for supplemental biotin in primary cell culture?

I am trying to overexpress a TurboID construct in mouse primary culture cells for validation experiments. Unfortunately the transfection of this construct appears to be killing my cells while...

27 February 2024 8,929 2 View

What is the best way to fix cells for non-permeabilized stains?

Hello, I need to stain some primary culture cells in permeabilized and non-permeabilized conditions to test if a protein is localizing to the exterior of the cell membrane. Our existing fixation...

21 September 2023 5,141 3 View

What is the stability of lysosomes in pfa-fixed cells?

Hello all, I'm currently designing experiments for a project to characterize lysosomes within primary culture astrocytes. One of the parameters I'm interested in is lysosomal localization and...

09 July 2021 1,808 3 View